Riboflavin is also called Vitamin B2. Riboflavin deficiency is called ariboflavinosis and is primarily caused by reduced dietary intake. Symptoms include sore throat, cheilosis, angular stomatitis, glossitis, corneal vascularization, dermatitis, and normocytic, normochromic anemia.
Riboflavin is quantitated in plasma using high performance liquid chromatography and tandem mass spectrometry. Testing of nonfasting specimens or the use of dietary vitamin B2 supplementation can result in elevated plasma vitamin B2 concentrations.
Reference interval is 1-19 ug/L.
Specimen requirement is a green top tube of blood. Specimen should be immediately centrifuged and the plasma transferred to an amber vial to protect from sunlight. It should be immediately frozen.